[Cat.# B015](Old Cat.# LAV013)F-PHFA KRAS Codon 12 and 13 Mutation Kit



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Primer mix solution and one each of 2 standard DNA plates (12 samples/kit)

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Overview

This product is a reagent kit for the detection of 13 different mutations seen in codons 12 and 13 of the KRAS gene from arbitrary human genomic DNA samples. It covers 7 common and 6 uncommon mutations. PCR is performed with the primer mix, which amplifies the gene regions of interest, and differences in base sequence between the PCR amplicons and fluorenscence-labeled double-stranded standard DNA are detected by FRET-based Preferential Homoduplex Formation Assay (F-PHFA).

*We can also provide customized kits for specific combinations of mutations. Please contact us for details.
*For Research Use Only. Not intended for diagnostic.

Features

• Detects mutations in KRAS codons 12 and 13 with a high sensitivity
• Analysis completed in only 2 steps (3.5 hours)
• Good data reproducibility, even with degraded genomic samples
• Capable of analyzing purified genomic DNA samples as small as 20 ng

*F-PHFA developed by RIKEN GENESIS is a Robust Technology for gene mutation analysis. F-PHFA minimizes data inaccuracy caused by manual operation errors in traditional methods.

Compatible devices

This kit has been confirmed to be compatible with following real-time PCR devices.
Bio-Rad CFX96 / ABI 7500 FastDx / Roche LightCycler 480 system II

*Please contact us to inquire about using this kit with other devices.

Mutations detected

Common mutations

Amino Acid Change Base Change
G12A GGT>GCT
G12C GGT>TGT
G12D GGT>GAT
G12R GGT>CGT
G12V GGT>GTT
G12S GGT>AGT
G13D GGC>GAC

Uncommon mutations

Amino Acid Change Base Change
G13C GGC>TGC
G13R GGC>CGC
G13S GGC>AGC
G13A GGC>GCC
G13V GGC>GTC
G12F GGT>TTT

Standard DNA plate

Each standard DNA plate can be used to analyze up to 12 samples (including one negative control). The F-PHFA reaction reagent is dried down into each well of the standard DNA plate, meaning that sample preparation simply consists of dispensing the PCR amplicons directly into the wells The standard DNA plates can be cut apart so that only the rows required for the number of samples.


Standard DNA plate 1


Standard DNA plate 2

Instructions for kit use

Analysis can be performed in only 3.5 hours, from the PCR reaction to detection, using a real-time PCR device.


*This kit does not include Taq polymerase or other PCR reaction reagents. Please use commercially available products. (Recommended product:TaKaRa Taq™ Hot Start Version)

Please contact us if you have any questions

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