LBx® Probe Probes for Liquid biopsy

LBx® Probe

  • 【2016.04.07】 Poster presentation in Molecular Diagnostic Europe 2016
  • 【2016.03.07】 Poster presentation in Molecular Medicine Tri-Con 2016
  • 【2016.03.06】 Product release of LBx® Probe

Outline

Patient derived fluid based molecular diagnostics, or Liquid Biopsy, is generating significant attention because cancer originating cell-free DNA found in the blood stream can indicate current tumor status. The highly sensitive and specific Digital PCR (dPCR) is one of the promising technologies for Liquid Biopsy and would provide an absolute count of target molecules. Here, we develop genetic mutation detection probe mixes specific for dPCR.

  • Very high target specificity giving minimal false positive results
  • Validated by dPCR
  • Small amplicon size

Format

20x probe/primer mix

A single tube for the 50 reactions (scale for 20 μl/reaction)

Three categories

  • Mutation Detection: 93 mutations
  • Copy Number : 23 genes
  • RNA Expression : 3 kinds

*at the time on 2016/03

Quality Control

  • Equipment:Droplet Digital PCR™ (QX200)
  • QC:10 copies of mutant plasmid in wild type Human gDNA

Seminar and Poster

  • A Development of BRAF, NRAS, MEK1 mutation screening assay as possible prognostic and predictive tools for metastatic melanoma using digital PCR platform, Molecular Diagnostics Europe 2016

Probe list

Gene Target Cat.# (Old Cat.#)
AKT1 E17K A084 (65119)
CNV A104 (65501)
AKT2 CNV A105 (65502)
ALK T1151ins A085 (65120)
L1152R A066 (65101)
C1156Y A067 (65102)
I1171T A068 (65103)
F1174L A069 (65104)
V1180L A070 (65105)
L1196M A071 (65106)
G1202R A072 (65107)
S1206Y A073 (65108)
G1269A A074 (65109)
Multi1 A089 (65125)
Multi2 A090 (65126)
Multi3 A091 (65127)
CNV A106 (65503)
L1198F A101 (65137)
AP3B1 (Reference) CNV A122 (65519)
APOB (Reference) CNV A123 (65520)
AQP5 (Reference) CNV A124 (65521)
AR V7 65703
CNV A109 (65506)
Multi1 -
BRAF Screen1 A095 (65131)
CNV 65527
CCND1 CNV A110 (65507)
CDK4 CNV A111 (65508)
CDK6 CNV A112 (65509)
cMET Exon14 Skipping -
CNV A108 (65505)
CTNNB1 CNV A113 (65510)
EGFR T790M A075 (65110)
C797S A083 (65118)
S492R1 A102 (65138)
S492R2 A103 (65139)
Multi2 A092 (65128)
CNV 65531
Multi3 -
ESR1 Fusion A128 (65702)
Y537N A078 (65113)
Y537S A079 (65114)
D538G A080 (65115)
E380Q A081 (65116)
Multi1 A082 (65117)
Screen1 A086 (65121)
CNV A120 (65517)
EZH2 Screen1 A100 (65136)
FGFR1 CNV A114 (65511)
FGFR2 CNV A115 (65512)
FGFR3 CNV A116 (65513)
HER2,ERBB2 CNV 65530
IDH1 Screen1 A097 (65133)
IDH2 Screen1 A098 (65134)
Screen2 A099 (65135)
IDO1 - -
IDO2 - -
IGF1R CNV 65528
IGF2R CNV 65529
KIT CNV A117 (65514)
KRAS CNV A107 (65504)
MEK1 Screen1 A094 (65130)
CNV 65524
NRAS Screen1 A096 (65132)
CNV 65525
PD-L1 - -
PD-L2 - -
PDGFRa CNV A118 (65515)
PIK3CA Screen1 A087 (65123)
Screen2 A088 (65124)
CNV A119 (65516)
PMP22 (Reference) CNV A125 (65522)
PTEN Screen1 -
Screen2 -
Screen3 -
ROS1 G2032R A077 (65112)
RPP30 (Reference) CNV A121 (65518)
SMO D473H A076 (65111)
TDO - -
TOP3A (Reference) CNV A126 (65523)
VEGFA CNV 65526

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