RIKEN GENESIS has developed a Robust Technology for gene mutation analysis named “F-PHFA (FRET-based Preferential Homoduplex Formation Assay)”, which minimizes data inaccuracy caused by manual operation errors in traditional methods. RIKEN GENESIS supplies various application kits by F-PHFA.
|KRAS Codon 12 and 13 Mutation Kit||Flyer|
|BRAF Mutation Kit||Flyer|
|BRAF/NRAS Mutation Kit||Flyer|
*We can provide customized kits
for specific combinations of mutations
F-PHFA is a method combining competitive hybridization and FRET (Fluorescence resonance energy transfer). The mild thermal gradient forms a specific, fully complementary homo-duplex, and even one base substitution in the PCR products would be detected with high sensitivity as well as good reproducibility.
[Example 1] Sensitivity and reproducibility data with F-PHFA KRAS Mutation Kit
A novel and simple KRAS mutation detection method, FRET-Based Preferential Homoduplex Formation Assay (f-PHFA): Results from a multicenter, clinical evaluation trial. J Clin Oncol 30, 2012 (suppl 4; abstr 476)
[Example 2] The threshold setting for F-PHFA KRAS Mutation Kit with clinical samples
|F-PHFA application kit is compatible with the following real time PCR equipment. Please contact us for the use of other PCR equipment.||○ Bio-Rad CFX96|
|○ ABI 7500 FastDx|
|○ Roche LightCycler 480 system II|
RIKEN GENESIS CO., LTD.
TEL: +81 45 521 8781 / FAX: +81 45 521 8786 / e-mail: firstname.lastname@example.org>